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Figure 6
Calculation of dissociation constants
(Kd) for the binding between 293- and CHO-derived glypican-AP fusion proteins against Type I collagen (A), bFGF (B), and laminin-1 (C). For each of the ACE electrophoretograms in Figure 4, retardation coefficients R’s were determined and plotted against ligand concentrations as described in Materials and Methods.  For each plot a Kd value was calculated. The results are shown in Figure 7.
 

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