Calculation of dissociation constants (Kd) for the binding between 293- and CHO-derived glypican-AP fusion
proteins against Type I collagen (A), bFGF (B), and laminin-1 (C). For each of the ACE
electrophoretograms in Figure 4, retardation coefficients Rs were determined and
plotted against ligand concentrations as described in Materials and Methods. For
each plot a Kd value was
calculated. The results are shown in Figure 7.