
Figure 2
Schematic diagram of ACE. The illustrations represent an ACE gel before and after
electrophoresis. A labeled PG, loaded into the top horizontal slot, migrates through
lanes containing the test ligand at various concentrations, embedded in 1% low melting
point agarose. The extent to which the PG migration is retarded, as a function of
ligand concentration, can be used
to calculate the dissociation constant K_{d} for the interaction. If the concentration of labeled PG is
much less than K_{d}, the value of K_{d} may be calculated from the equation R = R¥/(1+ K_{d}/[P]) where R is the retardation
coefficient, calculated as m,
the amount of retardation, divided by n, the distance that unimpeded PG travels, R¥ is the value of R approached at saturating ligand concentrations.
[P] is the concentration of the ligand embedded in each lane. Reprinted with the
permission of author (Herndon and Lander 1997).

